nsc34 murine motor neuron like cell line Search Results


nsc34 cells  (CELLutions Biosystems)
90
CELLutions Biosystems nsc34 cells
RRM1-C/S mutations unravel the pathogenic properties of TDP-43. A, RRM1-C/S substitution enhances the aggregation propensity of TDP-43 with familial ALS-linked mutations in the C-terminal domain. EGFP-fused WT or A315T and Q331K mutants of TDP-43, either with (d–f) or without (a–c) the C175S mutation, were transiently expressed in HEK293A cells. B, quantification of cells with aggregates. Data represent the mean percentages of cells harboring aggregates in total transfected cells ± S.E. (n = 8 images); 20–120 cells were counted in each image. *, p < 0.05 by one-way ANOVA of Bonferroni's test. C, cytosolic aggregates of TDP-43 with RRM1-C/S injure the motor neuron cell line. a–l, confocal micrographs showing subcellular distributions and inclusion formation of TDP-43-EGFP in <t>NSC34.</t> D, a and b, fluorometric assay for neurotoxicity of RRM1-C/S mutants in NSC-34 cells. Murine motor neuron NSC34 cells plated in a 96-well culture plate were transiently transfected with plasmids expressing TDP-43-FLAG (0.2 μg/well) of WT, ALS-linked mutants (D169G and A315T), or Cys-173/Cys-175 mutants (C173S, C175S, and C173S/C175S (DCS)) with or without mNLS. Data represent the mean ± S.E. (n = 12 wells from 4 independent experiments; each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Bonferroni's test. b, dead cell marker bis-AAF-R110 was also normalized to luciferase activity from co-transfected Renilla luciferase reporter plasmid. The Renilla luciferase reporter gene (0.02 μg/well) was co-transfected with pcDNA-TDP-43-FLAG. Data represent the mean ± S.E. (n = 11–12). Each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Dunnett's test.
Nsc34 Cells, supplied by CELLutions Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine motoneuron cell line  (ATCC)
94
ATCC murine motoneuron cell line
RRM1-C/S mutations unravel the pathogenic properties of TDP-43. A, RRM1-C/S substitution enhances the aggregation propensity of TDP-43 with familial ALS-linked mutations in the C-terminal domain. EGFP-fused WT or A315T and Q331K mutants of TDP-43, either with (d–f) or without (a–c) the C175S mutation, were transiently expressed in HEK293A cells. B, quantification of cells with aggregates. Data represent the mean percentages of cells harboring aggregates in total transfected cells ± S.E. (n = 8 images); 20–120 cells were counted in each image. *, p < 0.05 by one-way ANOVA of Bonferroni's test. C, cytosolic aggregates of TDP-43 with RRM1-C/S injure the motor neuron cell line. a–l, confocal micrographs showing subcellular distributions and inclusion formation of TDP-43-EGFP in <t>NSC34.</t> D, a and b, fluorometric assay for neurotoxicity of RRM1-C/S mutants in NSC-34 cells. Murine motor neuron NSC34 cells plated in a 96-well culture plate were transiently transfected with plasmids expressing TDP-43-FLAG (0.2 μg/well) of WT, ALS-linked mutants (D169G and A315T), or Cys-173/Cys-175 mutants (C173S, C175S, and C173S/C175S (DCS)) with or without mNLS. Data represent the mean ± S.E. (n = 12 wells from 4 independent experiments; each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Bonferroni's test. b, dead cell marker bis-AAF-R110 was also normalized to luciferase activity from co-transfected Renilla luciferase reporter plasmid. The Renilla luciferase reporter gene (0.02 μg/well) was co-transfected with pcDNA-TDP-43-FLAG. Data represent the mean ± S.E. (n = 11–12). Each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Dunnett's test.
Murine Motoneuron Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine nsc34  (Cedarlane)
95
Cedarlane murine nsc34
RRM1-C/S mutations unravel the pathogenic properties of TDP-43. A, RRM1-C/S substitution enhances the aggregation propensity of TDP-43 with familial ALS-linked mutations in the C-terminal domain. EGFP-fused WT or A315T and Q331K mutants of TDP-43, either with (d–f) or without (a–c) the C175S mutation, were transiently expressed in HEK293A cells. B, quantification of cells with aggregates. Data represent the mean percentages of cells harboring aggregates in total transfected cells ± S.E. (n = 8 images); 20–120 cells were counted in each image. *, p < 0.05 by one-way ANOVA of Bonferroni's test. C, cytosolic aggregates of TDP-43 with RRM1-C/S injure the motor neuron cell line. a–l, confocal micrographs showing subcellular distributions and inclusion formation of TDP-43-EGFP in <t>NSC34.</t> D, a and b, fluorometric assay for neurotoxicity of RRM1-C/S mutants in NSC-34 cells. Murine motor neuron NSC34 cells plated in a 96-well culture plate were transiently transfected with plasmids expressing TDP-43-FLAG (0.2 μg/well) of WT, ALS-linked mutants (D169G and A315T), or Cys-173/Cys-175 mutants (C173S, C175S, and C173S/C175S (DCS)) with or without mNLS. Data represent the mean ± S.E. (n = 12 wells from 4 independent experiments; each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Bonferroni's test. b, dead cell marker bis-AAF-R110 was also normalized to luciferase activity from co-transfected Renilla luciferase reporter plasmid. The Renilla luciferase reporter gene (0.02 μg/well) was co-transfected with pcDNA-TDP-43-FLAG. Data represent the mean ± S.E. (n = 11–12). Each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Dunnett's test.
Murine Nsc34, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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motor neuron nsc34 cells  (Cosmo Bio USA)
90
Cosmo Bio USA motor neuron nsc34 cells
RRM1-C/S mutations unravel the pathogenic properties of TDP-43. A, RRM1-C/S substitution enhances the aggregation propensity of TDP-43 with familial ALS-linked mutations in the C-terminal domain. EGFP-fused WT or A315T and Q331K mutants of TDP-43, either with (d–f) or without (a–c) the C175S mutation, were transiently expressed in HEK293A cells. B, quantification of cells with aggregates. Data represent the mean percentages of cells harboring aggregates in total transfected cells ± S.E. (n = 8 images); 20–120 cells were counted in each image. *, p < 0.05 by one-way ANOVA of Bonferroni's test. C, cytosolic aggregates of TDP-43 with RRM1-C/S injure the motor neuron cell line. a–l, confocal micrographs showing subcellular distributions and inclusion formation of TDP-43-EGFP in <t>NSC34.</t> D, a and b, fluorometric assay for neurotoxicity of RRM1-C/S mutants in NSC-34 cells. Murine motor neuron NSC34 cells plated in a 96-well culture plate were transiently transfected with plasmids expressing TDP-43-FLAG (0.2 μg/well) of WT, ALS-linked mutants (D169G and A315T), or Cys-173/Cys-175 mutants (C173S, C175S, and C173S/C175S (DCS)) with or without mNLS. Data represent the mean ± S.E. (n = 12 wells from 4 independent experiments; each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Bonferroni's test. b, dead cell marker bis-AAF-R110 was also normalized to luciferase activity from co-transfected Renilla luciferase reporter plasmid. The Renilla luciferase reporter gene (0.02 μg/well) was co-transfected with pcDNA-TDP-43-FLAG. Data represent the mean ± S.E. (n = 11–12). Each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Dunnett's test.
Motor Neuron Nsc34 Cells, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


RRM1-C/S mutations unravel the pathogenic properties of TDP-43. A, RRM1-C/S substitution enhances the aggregation propensity of TDP-43 with familial ALS-linked mutations in the C-terminal domain. EGFP-fused WT or A315T and Q331K mutants of TDP-43, either with (d–f) or without (a–c) the C175S mutation, were transiently expressed in HEK293A cells. B, quantification of cells with aggregates. Data represent the mean percentages of cells harboring aggregates in total transfected cells ± S.E. (n = 8 images); 20–120 cells were counted in each image. *, p < 0.05 by one-way ANOVA of Bonferroni's test. C, cytosolic aggregates of TDP-43 with RRM1-C/S injure the motor neuron cell line. a–l, confocal micrographs showing subcellular distributions and inclusion formation of TDP-43-EGFP in NSC34. D, a and b, fluorometric assay for neurotoxicity of RRM1-C/S mutants in NSC-34 cells. Murine motor neuron NSC34 cells plated in a 96-well culture plate were transiently transfected with plasmids expressing TDP-43-FLAG (0.2 μg/well) of WT, ALS-linked mutants (D169G and A315T), or Cys-173/Cys-175 mutants (C173S, C175S, and C173S/C175S (DCS)) with or without mNLS. Data represent the mean ± S.E. (n = 12 wells from 4 independent experiments; each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Bonferroni's test. b, dead cell marker bis-AAF-R110 was also normalized to luciferase activity from co-transfected Renilla luciferase reporter plasmid. The Renilla luciferase reporter gene (0.02 μg/well) was co-transfected with pcDNA-TDP-43-FLAG. Data represent the mean ± S.E. (n = 11–12). Each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Dunnett's test.

Journal: The Journal of Biological Chemistry

Article Title: Aberrant Assembly of RNA Recognition Motif 1 Links to Pathogenic Conversion of TAR DNA-binding Protein of 43 kDa (TDP-43) *

doi: 10.1074/jbc.M113.451849

Figure Lengend Snippet: RRM1-C/S mutations unravel the pathogenic properties of TDP-43. A, RRM1-C/S substitution enhances the aggregation propensity of TDP-43 with familial ALS-linked mutations in the C-terminal domain. EGFP-fused WT or A315T and Q331K mutants of TDP-43, either with (d–f) or without (a–c) the C175S mutation, were transiently expressed in HEK293A cells. B, quantification of cells with aggregates. Data represent the mean percentages of cells harboring aggregates in total transfected cells ± S.E. (n = 8 images); 20–120 cells were counted in each image. *, p < 0.05 by one-way ANOVA of Bonferroni's test. C, cytosolic aggregates of TDP-43 with RRM1-C/S injure the motor neuron cell line. a–l, confocal micrographs showing subcellular distributions and inclusion formation of TDP-43-EGFP in NSC34. D, a and b, fluorometric assay for neurotoxicity of RRM1-C/S mutants in NSC-34 cells. Murine motor neuron NSC34 cells plated in a 96-well culture plate were transiently transfected with plasmids expressing TDP-43-FLAG (0.2 μg/well) of WT, ALS-linked mutants (D169G and A315T), or Cys-173/Cys-175 mutants (C173S, C175S, and C173S/C175S (DCS)) with or without mNLS. Data represent the mean ± S.E. (n = 12 wells from 4 independent experiments; each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Bonferroni's test. b, dead cell marker bis-AAF-R110 was also normalized to luciferase activity from co-transfected Renilla luciferase reporter plasmid. The Renilla luciferase reporter gene (0.02 μg/well) was co-transfected with pcDNA-TDP-43-FLAG. Data represent the mean ± S.E. (n = 11–12). Each value is normalized to vector control in each experiment). *, p < 0.05 versus vector control by one-way ANOVA of Dunnett's test.

Article Snippet: Murine motor neuron cell line NSC34 cells (Cellutions Biosystems, Vancouver, Canada) were maintained in DMEM containing 10% FBS for growth.

Techniques: Mutagenesis, Transfection, Expressing, Plasmid Preparation, Marker, Luciferase, Activity Assay